IL-17A Rekombinanter Antikörper

IL-17A Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250413G7

Kat-Nr. : 98564-1-RR

Synonyme

Il17a, Interleukin 17A

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  FC experiment of Transfected HEK-293T using 98564-1-RR

  1x10^6 HEK-293T cells (left) and IL-17A transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat IL-17A Rabbit RecAb (98564-1-RR, Clone: 250413G7) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98564-1-RR

  1x10^6 HEK-293Tcells (left) and IL-17A transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat IL-17A Rabbit RecAb (98564-1-RR, Clone: 250413G7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98564-1-RR bindet in FC IL-17A und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-17A fusion protein Eg3339
• Vollständiger Name: similar to Interleukin-17 precursor (IL-17) (Cytotoxic T lymphocyte-associated antigen 8) (CTLA-8)
• Berechnetes Molekulargewicht: 17kDa
• Gene symbol: LOC301289
• Gene ID (NCBI): 301289
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

IL-17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.