IL-17A Rekombinanter Antikörper

IL-17A Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230038C7

Kat-Nr. : CL488-98005

Synonyme

Il17a, 230038C7, IL 17A, Il17, IL-17

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  FC experiment of C57BL/6 Th17-polarized splenocytes using CL488-98005

  1x10^6 untreated or PMA and ionomycin treated C57BL/6 Th17-polarized splenocytes were intracellularly stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.25 ug CoraLite® Plus 488 Anti-Mouse IL-17A Rabbit RecAb (CL488-98005, Clone:230038C7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA and ionomycin treated C57BL/6 Th17-polarized splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-98005 bindet in FC (Intra) IL-17A und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-17A fusion protein Eg0624
• Vollständiger Name: interleukin 17A
• Berechnetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: NM-010552
• Gene symbol: Il17a
• Gene ID (NCBI): 16171
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

IL-17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.