IL-17D Monoklonaler Antikörper

IL-17D Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D7H4

Kat-Nr. : CL488-66143

Synonyme

IL17D, interleukin 17D, IL-27, IL27, IL 27

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  FC experiment of human PBMCs using CL488-66143

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 488-conjugated IL-17D Monoclonal antibody (CL488-66143, Clone: 1D7H4) (red) or 0.25 ug CoraLite® Plus 488 Mouse IgG2b isotype control (11B8C4) (CL488-66360-3, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): humane PBMCs

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66143 bindet in FC (Intra) IL-17D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-17D fusion protein Ag6762
• Vollständiger Name: interleukin 17D
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC036243
• Gene symbol: IL-17D
• Gene ID (NCBI): 53342
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL17D is a secreted cytokine with homology to the IL-17 family of proteins. IL17D is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas. The treatment of endothelial cells with IL17D has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by IL17D was found to be NF-kappa B-dependent.