IL-2 Polyklonaler Antikörper

IL-2 Polyklonal Antikörper für FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

FC (Intra), ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30199-1-AP

Synonyme

Il2, interleukin 2

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  FC experiment of rat splenocytes using 30199-1-AP

  1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IL-2 (30199-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of rat splenocytes using 30199-1-AP

  1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IL-2 (30199-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, and 0.5 ug rabbit IgG isotype control, and 0.5 ug CoraLite®594 Anti-Rat CD8a (OX-8) (CL594-65180, Clone: OX-8). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA and ionomycin treated rat splenocytes cells

Empfohlene Verdünnung

• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30199-1-AP bindet in FC (Intra), ELISA IL-2 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-2 fusion protein Eg0260
• Vollständiger Name: interleukin 2
• Berechnetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: NM_053836
• Gene symbol: Il2
• Gene ID (NCBI): 116562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.