IL-23 p19 Rekombinanter Antikörper

IL-23 p19 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

242139G12

Kat-Nr. : 98392-1-RR

Synonyme

IL-23 subunit alpha, Il23a, IL-23A, IL-23-A, IL23P19

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  FC experiment of Transfected HEK-293T using 98392-1-RR

  1x10^6 HEK-293T cells(left) and IL23A -transfected HEK-293T cells(right) were stained with 0.25 ug Anti-Mouse IL23A Rabbit RecAb (98392-1-RR, Clone:242139G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Transfected HEK-293T using 98392-1-RR

  1x10^6 HEK-293T cells(left) and IL23A -transfected HEK-293T cells(right) were stained with 0.25 ug Anti-Mouse IL23A Rabbit RecAb (98392-1-RR, Clone:242139G12)(red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98392-1-RR bindet in FC (Intra) IL-23 p19 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-23 p19 fusion protein Eg1634
• Vollständiger Name: interleukin 23, alpha subunit p19
• Berechnetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_031252.2
• Gene symbol: Il23a
• Gene ID (NCBI): 83430
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).