IL-28A Rekombinanter Antikörper

IL-28A Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242247E2

Kat-Nr. : 98388-1-RR

Synonyme

Ifnl2, IFN-lambda-2, Il28a, Interferon lambda-2, Interleukin-28A

---

• 

  FC experiment of Transfected HEK-293T using 98388-1-RR

  1x10^6 HEK-293T cells(left) and Il28a-transfected HEK-293T cells(right) were stained with 0.25 ug Anti-Mouse IL-28A Rabbit RecAb (98388-1-RR, Clone:242247E2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98388-1-RR

  1x10^6 HEK-293T cells(left) and Il28a-transfected HEK-293T cells(right) were stained with 0.25 ug Anti-Mouse IL-28A Rabbit RecAb (98388-1-RR, Clone:242247E2)(red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98388-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98388-1-RR against Mouse IL-28A were performed. The affinity constant is 0.137 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98388-1-RR bindet in FC IL-28A und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-28A fusion protein Eg3169
• Vollständiger Name: interleukin 28A
• Berechnetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_001024673.2
• Gene symbol: Il28a
• Gene ID (NCBI): 330496
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

IL28A, also named as IFNL2 and ZCYT020, is a cytokine with immunomodulatory activity. It up-regulates MHC class I antigen expression. IL28A is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. It plays a significant role in the antiviral immune defense in the intestinal epithelium.