IL-3 Rekombinanter Antikörper

IL-3 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

240417E1

Kat-Nr. : 98363-1-RR

Synonyme

IL3, IL 3, Interleukin 3, Interleukin-3, Multipotential colony-stimulating factor

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  FC experiment of human PBMCs using 98363-1-RR

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-3 Rabbit RecAb (98363-1-RR, Clone: 240417E1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Treated human PBMCs were pre-stimulated with anti-human CD3/CD28 and recombinant human IL-2 and IL-4.

• 

  FC experiment of human PBMCs using 98363-1-RR

  1x10^6 PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-3 Rabbit RecAb (98363-1-RR, Clone: 240417E1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-3 Rabbit RecAb (98363-1-RR, Clone: 240417E1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Treated human PBMCs were pre-stimulated with anti-human CD3/CD28 and recombinant human IL-2 and IL-4.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA, Ionomycin and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98363-1-RR bindet in FC (Intra) IL-3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-3 fusion protein Eg0829
• Vollständiger Name: interleukin 3 (colony-stimulating factor, multiple)
• Berechnetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: NM_000588.3
• Gene symbol: IL-3
• Gene ID (NCBI): 3562
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. (PMID: 24103757;2698876;2809196)