IL-4 Rekombinanter Antikörper

IL-4 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Biotin

CloneNo.

240794E11

Kat-Nr. : Biotin-FcA98138

Synonyme

IL4, B-cell stimulatory factor 1, BSF-1, IL 4, interleukin 4

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  FC experiment of human PBMCs using Biotin-FcA98138

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Biotin Anti-Human IL-4 Rabbit RecAb (Biotin-FcA98138, Clone: 240794E11) and PE-conjugated streptavidin. Cells were co-stained with FcZero-rAb™ APC Anti-Human CD3 (OKT3) Rabbit IgG Recombinant Antibody (APC-FcA65569, Clone: OKT3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA, Ionomycin and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-FcA98138 bindet in FC (Intra) IL-4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-4 fusion protein Eg0104
• Vollständiger Name: interleukin 4
• Berechnetes Molekulargewicht: 153 aa, 17 kDa
• GenBank-Zugangsnummer: BC070123
• Gene symbol: IL-4
• Gene ID (NCBI): 3565
• Konjugation: Biotin
• Excitation/Emission maxima wavelengths: -
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.