IL-4 Monoklonaler Antikörper

IL-4 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Ratte / IgG1, kappa

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

CloneNo.

11B11

Kat-Nr. : CL488-65074

Synonyme

Il 4, Il4, IL-4, interleukin 4

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  FC experiment of Mouse Th2-polarized splenocytes using CL488-65074

  1X10^6 unstimulated or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) mouse Th2-polarized splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and then intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse IL-4 (CL488-65074, Clone:11B11).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA and ionomycin stimulated mouse Th2-polarized splenocytes
• Erfolgreiche Detektion in FC: PMA and ionomycin stimulated mouse Th2-polarized splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

CL488-65074 bindet in FC (Intra) IL-4 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Partially purified native mouse IL-4
• Vollständiger Name: interleukin 4
• GenBank-Zugangsnummer: BC027514
• Gene symbol: IL-4
• Gene ID (NCBI): 16189
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival. (PMID: 24489573;3049907;21663408)

Publikationen

Species	Application	Title
	FC	Int J Mol Sci; Development of a Vaccine Candidate Based on Surface-Displayed Particles of Mycobacterium tuberculosis from the MTB39A Protein; Authors - Pu Wang