IL-4 Rekombinanter Antikörper

IL-4 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

240794E11

Kat-Nr. : CL594-98138

Synonyme

IL4, 240794E11, B-cell stimulatory factor 1, BSF-1, IL 4

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  FC experiment of human PBMCs using CL594-98138

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 5 ul CoraLite®594 Anti-Human IL-4 Rabbit RecAb (CL594-98138, Clone: 240794E11). Cells were co-stained with CoraLite® Plus 488 Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (CL488-65570, Clone: UCHT1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA, Ionomycin and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98138 bindet in FC (Intra) IL-4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-4 fusion protein Eg0104
• Vollständiger Name: interleukin 4
• Berechnetes Molekulargewicht: 153 aa, 17 kDa
• GenBank-Zugangsnummer: BC070123
• Gene symbol: IL-4
• Gene ID (NCBI): 3565
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.