IL-4 Rekombinanter Antikörper

IL-4 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241249H7

Kat-Nr. : CL647-98255

Synonyme

241249H7, B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1

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  FC experiment of Transfected HEK-293T using CL647-98255

  1x10^6 untransfected or IL-4 transfected HEK-293T cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Rat IL-4 Rabbit RecAb (CL647-98255, Clone: 241249H7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using CL647-98255

  1x10^6 untransfected or IL-4 transfected HEK-293T cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Rat IL-4 Rabbit RecAb (CL647-98255, Clone: 241249H7) (red) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98255 bindet in FC (Intra) IL-4 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: IL-4 fusion protein Eg1113
• Vollständiger Name: interleukin 4
• Berechnetes Molekulargewicht: 16kDa
• GenBank-Zugangsnummer: NM_201270.1
• Gene symbol: IL-4
• Gene ID (NCBI): 287287
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.