IL-4 Monoklonaler Antikörper

IL-4 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Ratte / IgG1, kappa

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

PE Fluorescent Dye

Publikationen(1)

CloneNo.

11B11

Kat-Nr. : PE-65074

Synonyme

IL-4, interleukin 4, 11B11, Il 4, Il4

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  FC experiment of C57BL/6 Th2-polarized splenocytes using PE-65074

  1X10^6 unstimulated or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) Th2-polarized splenocytes were surface stained 0.2 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C), and then intracellularly stained with 0.125 ug PE Anti-Mouse IL-4 (PE-65074, Clone: 11B11).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): PMA and ionomycin stimulated Th2-polarized splenocytes
• Erfolgreiche Detektion in FC: PMA and ionomycin stimulated Th2-polarized splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

PE-65074 bindet in FC (Intra) IL-4 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Partially purified native mouse IL-4
• Vollständiger Name: interleukin 4
• GenBank-Zugangsnummer: BC027514
• Gene symbol: IL-4
• Gene ID (NCBI): 16189
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival. (PMID: 24489573;3049907;21663408)

Publikationen

Species	Application	Title
mouse	FC	J Ethnopharmacol; Daqing formula ameliorated allergic asthma and airway dysbacteriosis in mice challenged with ovalbumin and ampicillin; Authors - Li-Ren Wu