IL-6 Rekombinanter Antikörper

IL-6 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

240670D12

Kat-Nr. : 98087-1-RR

Synonyme

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

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  FC experiment of human PBMCs using 98087-1-RR

  1x10^6 untreated or LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

• 

  FC experiment of human PBMCs using 98087-1-RR

  1x10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL6 Rabbit Recombinant Antibody (98087-1-RR, Clone:240670D12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

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  Affinity and Kinetic Characterization of 98087-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98087-1-RR against Human IL-6 were performed. The affinity constant is 0.322 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): LPS and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98087-1-RR bindet in FC (Intra) IL-6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: interleukin 6 (interferon, beta 2)
• Berechnetes Molekulargewicht: 212 aa, 24 kDa
• GenBank-Zugangsnummer: BC015511
• Gene symbol: IL6
• Gene ID (NCBI): 3569
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.