IL-6 Rekombinanter Antikörper

IL-6 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240670D12

Kat-Nr. : CL647-98087

Synonyme

IL6, 240670D12, B-cell stimulatory factor 2, IFN-beta-2, IL 6

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  FC experiment of human PBMCs using CL647-98087

  1x10^6 untreated or LPS and Brefeldin A treated human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human IL-6 Rabbit RecAb (CL647-98087, Clone: 240670D12). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

• 

  FC experiment of human PBMCs using CL647-98087

  1x10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human IL-6 Rabbit RecAb (CL647-98087, Clone: 240670D12) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human IL-6 Rabbit RecAb (CL647-98087, Clone:240670D12) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): LPS and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98087 bindet in FC (Intra) IL-6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 6 (interferon, beta 2)
• Berechnetes Molekulargewicht: 212 aa, 24 kDa
• GenBank-Zugangsnummer: BC015511
• Gene symbol: IL6
• Gene ID (NCBI): 3569
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.