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IL-1 beta Monoklonaler Antikörper

IL-1 beta Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen(102)

CloneNo.

2A1B4

Kat-Nr. : 66737-1-Ig

Synonyme

2A1B4, Catabolin, IL 1, IL 1 beta, IL1 beta



Geprüfte Anwendungen

Erfolgreiche Detektion in WBTHP-1-Zellen, A431-Zellen, U-87 MG-Zellen
Erfolgreiche Detektion in IHChumanes Lungenkarzinomgewebe, humanes Hautkrebsgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCmit LPS behandelte THP-1-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66737-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA, Cell treatment IL-1 beta und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Kaninchen, Rind
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen IL-1 beta fusion protein Ag26030
Vollständiger Name interleukin 1, beta
Berechnetes Molekulargewicht 269 aa, 31 kDa
Beobachtetes Molekulargewicht 35 kDa
GenBank-ZugangsnummerBC008678
Gene symbol IL1B
Gene ID (NCBI) 3553
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IL-1 beta antibody 66737-1-IgProtokoll herunterladen
IHC protocol for IL-1 beta antibody 66737-1-IgProtokoll herunterladenl
IF protocol for IL-1 beta antibody 66737-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Metab

Lighting up arginine metabolism reveals its functional diversity in physiology and pathology

Authors - Rui Li
IF

Adv Mater

Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment

Authors - Yuewen Zhai
humanIF

Biomaterials

Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.

Authors - Angelina D Schoenenberger
IHC

J Nanobiotechnology

Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression

Authors - Yunhao Wang
WB,IF

J Nanobiotechnology

Implantation of biomimetic polydopamine nanocomposite scaffold promotes optic nerve regeneration through modulating inhibitory microenvironment

Authors - Tonghe Pan
WB,IHC

Cell Mol Life Sci

circACTA2 inhibits NLRP3 inflammasome-mediated inflammation via interacting with NF-κB in vascular smooth muscle cells

Authors - Yang Bai