- Featured Product
- KD/KO Validated
IL-1 beta Monoklonaler Antikörper
IL-1 beta Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human und mehr (2)
Anwendung
WB, IHC, IF/ICC, IP, ELISA, Cell treatment
Konjugation
Unkonjugiert
CloneNo.
2A1B4
Kat-Nr. : 66737-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | THP-1-Zellen, A431-Zellen, U-87 MG-Zellen | 
| Erfolgreiche Detektion in IHC | humanes Lungenkarzinomgewebe,  humanes Hautkrebsgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF/ICC | mit LPS behandelte THP-1-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| Immunhistochemie (IHC) | IHC : 1:50-1:500 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:1000-1:4000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below | 
| WB | See 83 publications below | 
| IHC | See 13 publications below | 
| IF | See 11 publications below | 
| IP | See 1 publications below | 
| ELISA | See 2 publications below | 
Produktinformation
66737-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA, Cell treatment IL-1 beta und zeigt Reaktivität mit human
| Getestete Reaktivität | human | 
| In Publikationen genannte Reaktivität | human, Kaninchen, Rind | 
| Wirt / Isotyp | Maus / IgG1 | 
| Klonalität | Monoklonal | 
| Typ | Antikörper | 
| Immunogen | IL-1 beta fusion protein Ag26030 | 
| Vollständiger Name | interleukin 1, beta | 
| Berechnetes Molekulargewicht | 269 aa, 31 kDa | 
| Beobachtetes Molekulargewicht | 35 kDa | 
| GenBank-Zugangsnummer | BC008678 | 
| Gene symbol | IL1B | 
| Gene ID (NCBI) | 3553 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Protein-G-Reinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for IL-1 beta antibody 66737-1-Ig | Protokoll herunterladen | 
| IHC protocol for IL-1 beta antibody 66737-1-Ig | Protokoll herunterladenl | 
| IF protocol for IL-1 beta antibody 66737-1-Ig | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Cell Metab Lighting up arginine metabolism reveals its functional diversity in physiology and pathology | ||
| Adv Mater Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment | ||
| Biomaterials Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo. | ||
| J Nanobiotechnology Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression | ||
| J Nanobiotechnology Implantation of biomimetic polydopamine nanocomposite scaffold promotes optic nerve regeneration through modulating inhibitory microenvironment | ||
| Cell Mol Life Sci circACTA2 inhibits NLRP3 inflammasome-mediated inflammation via interacting with NF-κB in vascular smooth muscle cells | 











