IL-12/IL-23 p40 Polyklonaler Antikörper

IL-12/IL-23 p40 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 17645-1-AP

Synonyme

CLMF, CLMF p40, CLMF2, IL 12 subunit p40, IL 12B, IL-12/IL-23 p40, IL12B, IL-12B, Interleukin 12 subunit beta, NKSF, NKSF2

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Galerie der Validierungsdaten

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  WB analysis using 17645-1-AP

  Recombinant protein IL-12B (Eg0431), IL-12 (Eg0575), IL-12A (Ag17625) were subjected to SDS PAGE followed by western blot with 17645-1-AP (IL-12B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

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  WB analysis of Recombinant protein using 17645-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 17645-1-AP (IL-12B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

17645-1-AP bindet in WB, IF, ELISA IL-12/IL-23 p40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-12/IL-23 p40 fusion protein Ag11837
• Vollständiger Name: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
• Berechnetes Molekulargewicht: 328 aa, 37 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC067498
• Gene symbol: IL12B
• Gene ID (NCBI): 3593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

Publikationen

Species	Application	Title
human	IF	Int J Pharm; Dual-targeting Tumor Cells and Tumor Associated Macrophages with Lipid Coated Calcium Zoledronate for Enhanced Lung Cancer Chemoimmunotherapy.; Authors - Xinlong Zang
human	WB	Cell Signal; Tumor-secreted GRP78 induces M2 polarization of macrophages by promoting lipid catabolism; Authors - Jinmiao Tian