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IL-12/IL-23 p40 Polyklonaler Antikörper

IL-12/IL-23 p40 Polyklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 17645-1-AP

Synonyme

IL12B, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12 P40, IL 12 subunit p40



Geprüfte Anwendungen

Erfolgreiche Detektion in WBRecombinant protein, HeLa-Zellen
Erfolgreiche Detektion in FC (Intra)Jurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 2 publications below
IFSee 2 publications below

Produktinformation

17645-1-AP bindet in WB, IF, FC (Intra), ELISA IL-12/IL-23 p40 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen IL-12/IL-23 p40 fusion protein Ag11837
Vollständiger Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Berechnetes Molekulargewicht 328 aa, 37 kDa
Beobachtetes Molekulargewicht45 kDa
GenBank-ZugangsnummerBC067498
Gene symbol IL12B
Gene ID (NCBI) 3593
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IL-12/IL-23 p40 antibody 17645-1-APProtokoll herunterladen
FC protocol for IL-12/IL-23 p40 antibody 17645-1-APDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Int J Pharm

Dual-targeting Tumor Cells and Tumor Associated Macrophages with Lipid Coated Calcium Zoledronate for Enhanced Lung Cancer Chemoimmunotherapy.

Authors - Xinlong Zang
humanWB

Cell Signal

Tumor-secreted GRP78 induces M2 polarization of macrophages by promoting lipid catabolism

Authors - Jinmiao Tian

Surgery

Sodium-glucose co-transporter 2 inhibitor canagliflozin modulates myocardial metabolism and inflammation in a swine model for chronic myocardial ischemia

Authors - Dwight D Harris
mouseWB,IF

Exploration (Beijing)

Bioengineer mesenchymal stem cell for treatment of glioma by IL-12 mediated microenvironment reprogramming and nCD47-SLAMF7 mediated phagocytosis regulation of macrophages

Authors - Man Li