IL-17RB Polyklonaler Antikörper

IL-17RB Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 20673-1-AP

Synonyme

IL17RB, IL-17 receptor homolog 1, IL 17Rh1, IL 17RB, EVI27

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  WB analysis using 20673-1-AP

  Human testis tissue was subjected to SDS PAGE followed by western blot with 20673-1-AP (IL-17RB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20673-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20673-1-AP (IL-17RB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20673-1-AP (IL17RB antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20673-1-AP (IL17RB antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20673-1-AP (IL-17RB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20673-1-AP (IL-17RB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20673-1-AP (IL17RB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20673-1-AP (IL17RB antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 20673-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IL-17RB antibody (20673-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Mammakarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• ELISA: See 1 publications below

Produktinformation

20673-1-AP bindet in WB, IHC, IF/ICC, ELISA IL-17RB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: interleukin 17 receptor B
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56-60 kDa
• GenBank-Zugangsnummer: NM_018725
• Gene symbol: IL-17RB
• Gene ID (NCBI): 55540
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL17RB, also named as CRL4, EVI27 and IL17BR, is a receptor for the proinflammatory cytokines IL17B and IL17E. IL17RB may play a role in controlling the growth and/or differentiation of hematopoietic cells. IL17RB has two isoforms with MW 56 kDa (isoform 1) and 32 kDa (isoform 2). The antibody is specific to IL17RB.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Induction of IL-25 secretion from tumour-associated fibroblasts suppresses mammary tumour metastasis.; Authors - Shu-Yi Yin
human	WB,IF	Cell Rep; Schwann cell promotes macrophage recruitment through IL-17B/IL-17RB pathway in injured peripheral nerves; Authors - Yanju Huang
human	IF	Respir Res; IL-25 induces airway remodeling in asthma by orchestrating the phenotypic changes of epithelial cell and fibrocyte; Authors - Xiujuan Yao
human	WB,IF	Front Immunol; Interleukin-25-Mediated-IL-17RB Upregulation Promotes Cutaneous Wound Healing in Diabetic Mice by Improving Endothelial Cell Functions.; Authors - Fang Zhang
	WB	Arch Toxicol; Fluoride induces apoptosis and autophagy through the IL-17 signaling pathway in mice hepatocytes.; Authors - Yangfei Zhao
human	WB	mBio; Viral Mimicry of Interleukin-17A by SARS-CoV-2 ORF8.; Authors - Xin Wu