IL-1 Beta Polyklonaler Antikörper

IL-1 Beta Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (5)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

585

Rezensionen (2)

Kat-Nr. : 16806-1-AP

Synonyme

IL1 beta, Catabolin, IL 1, IL 1 beta, IL1 BETA, IL-1 beta, IL1- beta, IL1B, IL1beta, IL1F2, IL-1β, Interleukin 1 beta, interleukin 1, beta, Pro IL-1β

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Galerie der Validierungsdaten

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  WB analysis using 16806-1-AP

  Non-treated THP-1 cells, THP-1 cells (treated with 100 ng/ml LPS for 3h, then added 300 ng/ml protein transport inhibitor for 3h) were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL-1 Beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16806-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL1 beta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 16806-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 16806-1-AP (IL1 beta antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 16806-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16806-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL-1 Beta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16806-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16806-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16806-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 16806-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16806-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 16806-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16806-1-AP (IL1 beta antibody at dilution of 1:200 (under 40x lens).

• 

  FC experiment of THP-1 using 16806-1-AP

  1X10^6 THP-1 cells untreated (dashed lines), LPS treated (red) were intracellularly stained with 0.4 ug Anti-Human IL-1 Beta (16806-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit LPS und Proteintransportinhibitor behandelte THP-1-Zellen, A431-Zellen, humanes Nebennierengewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: mit LPS behandelte THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 484 publications below
• IHC: See 96 publications below
• IF: See 37 publications below
• ELISA: See 4 publications below

Produktinformation

16806-1-AP bindet in WB, IHC, IF, FC, ELISA IL-1 Beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Kaninchen, Rind, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-1 Beta fusion protein Ag10295
• Vollständiger Name: interleukin 1, beta
• Berechnetes Molekulargewicht: 269 aa, 31 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC008678
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

Publikationen

Species	Application	Title
human	WB	Immunity; Omega-3 fatty acids prevent inflammation and metabolic disorder through inhibition of NLRP3 inflammasome activation.; Authors - Yiqing Yan
zebrafish	IF	Cell; Migratory Neural Crest Cells Phagocytose Dead Cells in the Developing Nervous System.; Authors - Yunlu Zhu
	WB,IF	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun
zebrafish	IF	Nat Commun; Dynamic control of proinflammatory cytokines Il-1β and Tnf-α by macrophages in zebrafish spinal cord regeneration.; Authors - Themistoklis M Tsarouchas
		Nat Commun; Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells.; Authors - Akiko Takahashi
human	WB	Gut Microbes; Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway; Authors - Jian-Wei Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Margarita (Verified Customer) (06-06-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: PC3 cells

IL-1 Beta Antibody Immunofluorescence validation (1:200 dilution) in PC3 cells (Cat no:16806-1-AP)

FH

Margarita (Verified Customer) (06-22-2022)

Secondary antibody: Anti-Rabbit AF 594

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: PC3 cells

IL-1 Beta Antibody Immunofluorescence validation (1:50 dilution) in PC3 cells (Cat no:16806-1-AP)

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