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IL-1 beta Polyklonaler Antikörper

IL-1 beta Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (6)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(888)

Kat-Nr. : 16806-1-AP

Synonyme

Catabolin, IL 1, IL 1 beta, IL1 beta, IL1- beta



Geprüfte Anwendungen

Erfolgreiche Detektion in WBmit LPS und Proteintransportinhibitor behandelte THP-1-Zellen, A431-Zellen, humanes Nebennierengewebe
Erfolgreiche Detektion in IHChumanes Tonsillitisgewebe, humanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCmit LPS behandelte THP-1-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16806-1-AP bindet in WB, IHC, IF/ICC, ELISA IL-1 beta und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Ente, Hausschwein, Hund, Kaninchen, Rind, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen IL-1 beta fusion protein Ag10295
Vollständiger Name interleukin 1, beta
Berechnetes Molekulargewicht 269 aa, 31 kDa
Beobachtetes Molekulargewicht 30-35 kDa
GenBank-ZugangsnummerBC008678
Gene symbol IL1B
Gene ID (NCBI) 3553
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for IL-1 beta antibody 16806-1-APProtokoll herunterladen
IHC protocol for IL-1 beta antibody 16806-1-APProtokoll herunterladenl
IF protocol for IL-1 beta antibody 16806-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Immunity

Omega-3 fatty acids prevent inflammation and metabolic disorder through inhibition of NLRP3 inflammasome activation.

Authors - Yiqing Yan
zebrafishIF

Cell

Migratory Neural Crest Cells Phagocytose Dead Cells in the Developing Nervous System.

Authors - Yunlu Zhu
WB

Trends Biotechnol

Chairside live biotherapeutic hydrogel for comprehensive periodontitis therapy

Authors - Kaifeng Li
humanWB

Nat Commun

Cell enlargement modulated by GATA4 and YAP instructs the senescence-associated secretory phenotype

Authors - Joae Joung
WB,IF

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
zebrafishIF

Nat Commun

Dynamic control of proinflammatory cytokines Il-1β and Tnf-α by macrophages in zebrafish spinal cord regeneration.

Authors - Themistoklis M Tsarouchas

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Margarita (Verified Customer) (06-06-2023)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: PC3 cells
IL-1 beta Antibody Immunofluorescence validation (1:200 dilution) in PC3 cells (Cat no:16806-1-AP)
FH

Margarita (Verified Customer) (06-22-2022)

Secondary antibody: Anti-Rabbit AF 594

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: PC3 cells
IL-1 beta Antibody Immunofluorescence validation (1:50 dilution) in PC3 cells (Cat no:16806-1-AP)