IL-1 beta Polyklonaler Antikörper

IL-1 beta Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 30234-1-AP

Synonyme

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

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  WB analysis using 30234-1-AP

  Untreated, LPS and Protein transport inhibitor treated THP-1 cells were subjected to SDS PAGE followed by western blot with 30234-1-AP (IL1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-87 MG using 30234-1-AP

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 30234-1-AP (IL1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

30234-1-AP bindet in WB, IF, ELISA IL-1 beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-1 beta fusion protein Ag30574
• Vollständiger Name: interleukin 1, beta
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: NM_000576
• Gene symbol: IL1B
• Gene ID (NCBI): 3553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets

Publikationen

Species	Application	Title
mouse,rat	IF	Turk J Med Sci; Vitamin D3 alleviates intestinal injury in necrotizing enterocolitis and lipopolysaccharide-induced inflammatory response in dendritic cells in rats; Authors - Bo Ke