IL-1-RAPL-1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IL-1-RAPL-1 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 21609-1-AP

Synonyme

IL1RAPL1, EC:3.2.2.6, IL 1 RAPL 1, IL 1RAPL 1, IL1R8

---

• 

  WB analysis using 21609-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21609-1-AP (IL-1-RAPL-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 21609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21609-1-AP (IL-1-RAPL-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21609-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21609-1-AP (IL-1-RAPL-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 21609-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21609-1-AP (IL-1-RAPL-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 21609-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21609-1-AP (IL-1-RAPL-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 21609-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using IL-1-RAPL-1 antibody (21609-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

21609-1-AP bindet in WB, IHC, IF-P, ELISA IL-1-RAPL-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-1-RAPL-1 fusion protein Ag16252
• Vollständiger Name: interleukin 1 receptor accessory protein-like 1
• Berechnetes Molekulargewicht: 696 aa, 80 kDa
• Beobachtetes Molekulargewicht: 80-100 kDa
• GenBank-Zugangsnummer: BC126345
• Gene symbol: IL-1-RAPL-1
• Gene ID (NCBI): 11141
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell; AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.; Authors - Hana Cho ; KD Validated
		Allergy; Skewed inflammation is associated with aberrant interleukin-37 signaling pathway in atopic dermatitis.; Authors - Tianheng Hou
human	WB	EMBO J; Splice-dependent trans-synaptic PTPδ-IL1RAPL1 interaction regulates synapse formation and non-REM sleep.; Authors - Haram Park
human	WB	Cell Death Dis; IL-37 isoform D downregulates pro-inflammatory cytokines expression in a Smad3-dependent manner.; Authors - Mingsheng Zhao ; KD Validated
mouse,human	WB,IF	J Neurosci; The X-linked intellectual disability protein IL1RAPL1 regulates dendrite complexity.; Authors - Caterina Montani ; KO Validated
		Cell Rep; IL-38 Ameliorates Skin Inflammation and Limits IL-17 Production from γδ T Cells.; Authors - Yingying Han