ST2 Polyklonaler Antikörper

ST2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

32

Kat-Nr. : 11920-1-AP

Synonyme

DER4, FIT 1, IL1RL1, IL-1RL1, IL-33R, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1

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Galerie der Validierungsdaten

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  WB analysis using 11920-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11920-1-AP (ST2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11920-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11920-1-AP (ST2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11920-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11920-1-AP (ST2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11920-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11920-1-AP (ST2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11920-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11920-1-AP (ST2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11920-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11920-1-AP (ST2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11920-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11920-1-AP (ST2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Lungenkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 14 publications below
• IF: See 11 publications below

Produktinformation

11920-1-AP bindet in WB, IHC, IF, ELISA ST2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ST2 fusion protein Ag2555
• Vollständiger Name: interleukin 1 receptor-like 1
• Berechnetes Molekulargewicht: 63 kDa, 37 kDa, 30 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC030975
• Gene symbol: IL1RL1
• Gene ID (NCBI): 9173
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)

Publikationen

Species	Application	Title
human	IHC	Proc Natl Acad Sci U S A; IL-33 activates tumor stroma to promote intestinal polyposis.; Authors - Rebecca L Maywald
human	IHC,IF	J Invest Dermatol; Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.; Authors - Yi-Giien Tsai
human	WB,IF	Oncogene; The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis.; Authors - Quan Zhou
human,mouse	WB,IHC	Am J Pathol; Cross Talk between ST2 and Transforming Growth Factor-β Receptor Signaling Promotes Renal Fibrosis; Authors - Xingxing Zhu
rat,mouse	WB,IHC,IF	J Neuroinflammation; Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3.; Authors - Hao-Nan Li
rat	WB	J Neuroinflammation; IL-33/ST2 signaling contributes to radicular pain by modulating MAPK and NF-κB activation and inflammatory mediator expression in the spinal cord in rat models of noncompressive lumber disk herniation.; Authors - Si-Jian Huang

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