IL-20RB Polyklonaler Antikörper

IL-20RB Polyklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 20521-1-AP

Synonyme

DIRS1, FNDC6, IL 20 receptor subunit beta, IL 20R beta, IL 20R2, IL 20RB, IL20RB, IL-20RB, interleukin 20 receptor beta

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 20521-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20521-1-AP (IL20RB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20521-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20521-1-AP (IL20RB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20521-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20521-1-AP (IL20RB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Apoptosised HeLa using 20521-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 20521-1-AP (IL20RB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20521-1-AP

  Immunofluorescent analysis of HepG2 cells, using IL20RB antibody 20521-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).

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  FC experiment of K-562 using 20521-1-AP

  1X10^6 K-562 cells were stained with 0.2ug IL20RB antibody (20521-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, Apoptotisierte HeLa-Zellen, HeLa-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

20521-1-AP bindet in WB, IF, FC, ELISA IL-20RB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-20RB fusion protein Ag14368
• Vollständiger Name: interleukin 20 receptor beta
• Berechnetes Molekulargewicht: 311 aa, 35 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 66-70 kDa
• GenBank-Zugangsnummer: BC033292
• Gene symbol: IL20RB
• Gene ID (NCBI): 53833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-20 receptor subunit beta (IL20RB) is a single-pass type I membrane protein of the type II cytokine receptor family. The interleukin-20-receptor I complex (IL-20-RI) is composed of two chains, IL20RA and IL20RB. IL-20-RI is a receptor for IL-19, IL-20 and IL-24. IL20RB can also form a heterodimer with IL22RA1. The IL22RA1/IL20RB dimer is a receptor for IL20 and IL24.

Publikationen

Species	Application	Title
human	FC	Front Immunol; Integrative single-cell transcriptomic investigation unveils long non-coding RNAs associated with localized cellular inflammation in psoriasis; Authors - Yuge Gao
human,mouse	WB,IF	J Transl Med; IL20RB signaling enhances stemness and chemotherapy resistance in pancreatic cancer; Authors - Xiao-Hui Li