IL-21R Polyklonaler Antikörper

IL-21R Polyklonal Antikörper für FC, IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10533-1-AP

Synonyme

CD360, IL 21 receptor, IL 21R, IL21R, IL-21R, interleukin 21 receptor, NILR, Novel interleukin receptor

---

Galerie der Validierungsdaten

• 

  WB analysis using 10533-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10533-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10533-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10533-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10533-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 10533-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10533-1-AP (IL21R Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 10533-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10533-1-AP (IL21R Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of COLO 320 using 10533-1-AP

  Immunofluorescent analysis of COLO 320 cells using 10533-1-AP (IL21R antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of K-562 using 10533-1-AP

  1X10^6 K-562 cells were stained with 0.2ug IL21R antibody (10533-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, K-562-Zellen, Maus-Thymusgewebe, Raji-Zellen, Ratten-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: COLO 320-Zellen
• Erfolgreiche Detektion in FC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

10533-1-AP bindet in WB, IHC, IF, FC, ELISA IL-21R und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-21R fusion protein Ag0762
• Vollständiger Name: interleukin 21 receptor
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC004348
• Gene symbol: IL21R
• Gene ID (NCBI): 50615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IL21R, a receptor for interleukin-21, belongs to the type I cytokine receptor family. IL21R has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3.

Publikationen

Species	Application	Title
human	IHC	J Immunother Cancer; Prognostic value of novel immune-related genomic biomarkers identified in head and neck squamous cell carcinoma.; Authors - Yao Yao
human	WB,IF	Stem Cells Int; Interleukin 21 Receptor Affects Adipogenesis of Human Adipose-Derived Stem/Stromal Cells.; Authors - Bruna Cristina Falavinha
rat	WB,IF	Int Immunopharmacol; Role of Interleukin-21 in retinal ischemia-reperfusion injury: Unveiling the impact on retinal ganglion cell apoptosis; Authors - Xue Zhang