IL-22RA2 Monoklonaler Antikörper

IL-22RA2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

5G11A9

Kat-Nr. : 66190-1-Ig

Synonyme

CRF2 10, CRF2 S1, CRF2X, IL 22 receptor subunit alpha 2, IL 22BP, IL 22R alpha 2, IL 22RA2, IL22BP, IL22RA2, IL-22RA2, Interleukin 22 binding protein, ZcytoR16

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Galerie der Validierungsdaten

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  WB analysis of Raji using 66190-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 66190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 66190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 66190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66190-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-22RA2 antibody (66190-1-Ig, Clone: 5G11A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 66190-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-22RA2 antibody (66190-1-Ig, Clone: 5G11A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

66190-1-Ig bindet in WB, IHC, IF, FC, ELISA IL-22RA2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-22RA2 fusion protein Ag18682
• Vollständiger Name: interleukin 22 receptor, alpha 2
• Berechnetes Molekulargewicht: 263 aa, 31 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC125167
• Gene symbol: IL22RA2
• Gene ID (NCBI): 116379
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-22 receptor subunit alpha-2 (IL22RA2) is a soluble member of the type II cytokine receptor family. Through binding to IL22, IL22RA2 prevents the interaction of IL22 with the functional IL22-R complex and blocks the activity of IL22 (in vitro). IL22RA2 may play an important role as an IL22 antagonist in the regulation of inflammatory responses. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Publikationen

Species	Application	Title
pig	WB,IHC	Front Cell Infect Microbiol; Oral Administration of Lactobacillus rhamnosus GG Ameliorates Salmonella Infantis-Induced Inflammation in a Pig Model via Activation of the IL-22BP/IL-22/STAT3 Pathway.; Authors - Gui-Yan Yang
human	FC	Cells; The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis.; Authors - Paloma Gómez-Fernández
human	WB,IF	Front Immunol; Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response.; Authors - Paloma Gómez-Fernández

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