- Featured Product
- KD/KO Validated
IL-6 Polyklonaler Antikörper
IL-6 Polyklonal Antikörper für WB, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (5)
Anwendung
WB, IHC, IP, Neutralization, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 21865-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | mit LPS und Proteintransportinhibitor behandelte HUVEC-Zellen | 
| Erfolgreiche IP | mit LPS und Proteintransportinhibitor behandelte HUVEC-Zellen | 
| Erfolgreiche Detektion in IF/ICC | mit LPS und Brefeldin A behandelte HUVEC-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 5 publications below | 
| WB | See 118 publications below | 
| IHC | See 188 publications below | 
| IF | See 38 publications below | 
| ELISA | See 5 publications below | 
Produktinformation
21865-1-AP bindet in WB, IHC, IP, Neutralization, ELISA IL-6 und zeigt Reaktivität mit human
| Getestete Reaktivität | human | 
| In Publikationen genannte Reaktivität | human, Hausschwein, Hund, Kaninchen, Rind, Ziege | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | IL-6 fusion protein Ag10565 | 
| Vollständiger Name | interleukin 6 (interferon, beta 2) | 
| Berechnetes Molekulargewicht | 212 aa, 24 kDa | 
| Beobachtetes Molekulargewicht | 20 kDa | 
| GenBank-Zugangsnummer | BC015511 | 
| Gene symbol | IL6 | 
| Gene ID (NCBI) | 3569 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for IL-6 antibody 21865-1-AP | Protokoll herunterladen | 
| IF protocol for IL-6 antibody 21865-1-AP | Protokoll herunterladen | 
| IP protocol for IL-6 antibody 21865-1-AP | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
| J Hematol Oncol FFAR2 expressing myeloid-derived suppressor cells drive cancer immunoevasion | ||
| Cancer Cell Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation. | ||
| Bioact Mater Genetically engineered M2-like macrophage-derived exosomes for P. gingivalis-suppressed cementum regeneration: From mechanism to therapy | ||
| Bioact Mater A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds | ||
| ACS Cent Sci Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Udesh (Verified Customer) (04-19-2023) | Lowest most band corresponds to IL-6 
  | 
| FH Elizabeth (Verified Customer) (12-03-2018) | 
 | 


