CD126/IL-6R alpha Monoklonaler Antikörper

CD126/IL-6R alpha Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2A5F4

Kat-Nr. : 66855-1-Ig

Synonyme

CD126, IL-6R alpha, 2A5F4, IL 6R alpha, IL 6R 1

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  WB analysis of human spleen using 66855-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66855-1-Ig (IL6R alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood leukocyte using 66855-1-Ig

  Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66855-1-Ig (IL6R alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66855-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66855-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66855-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66855-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Milzgewebe, Human peripheral blood leukocyte
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

66855-1-Ig bindet in WB, IHC, IF, ELISA CD126/IL-6R alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD126/IL-6R alpha fusion protein Ag18263
• Vollständiger Name: interleukin 6 receptor
• Berechnetes Molekulargewicht: 468 aa, 52 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC132684
• Gene symbol: IL-6R alpha
• Gene ID (NCBI): 3570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).

Publikationen

Species	Application	Title
human	IF	Front Immunol; Expression and clinical significance of interleukin-6 pathway in cholangiocarcinoma; Authors - Dongqing Gu