gp130 Monoklonaler Antikörper

gp130 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1H7C5

Kat-Nr. : 67766-1-Ig

Synonyme

CD130, CDw130, Glycoprotein 130, GP130, GP130 RAPS, IL 6 receptor subunit beta, IL 6R beta, IL 6R subunit beta, IL 6RB, IL6R beta, IL-6RB, IL6ST, Membrane glycoprotein 130

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Galerie der Validierungsdaten

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  WB analysis using 67766-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67766-1-Ig (gp130 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 67766-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67766-1-Ig (gp130 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 67766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67766-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67766-1-Ig (gp130 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, DU 145-Zellen, NCCIT-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

67766-1-Ig bindet in WB, IP, IHC, IF, ELISA gp130 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: gp130 fusion protein Ag28808
• Vollständiger Name: interleukin 6 signal transducer (gp130, oncostatin M receptor)
• Berechnetes Molekulargewicht: 104 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: NM_002184
• Gene symbol: IL6ST
• Gene ID (NCBI): 3572
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC) (PMID: 9143707; 20610800). These cytokines signal through the gp130/Jak/STAT pathway (PMID: 9716487). Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells (PMID: 9815830).

Publikationen

Species	Application	Title
human	WB,IF,IP	Cell Prolif; Increased SPON1 promotes pancreatic ductal adenocarcinoma progression by enhancing IL-6 trans-signalling.; Authors - Yanmiao Huo
human	IHC	Cancer Med; Construction of a prognostic model for triple-negative breast cancer based on immune-related genes, and associations between the tumor immune microenvironment and immunological therapy; Authors - Yue Zhu