INS Polyklonaler Antikörper

INS Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(89)

Rezensionen (2)

Kat-Nr. : 15848-1-AP

Synonyme

insulin

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  IHC staining of mouse pancreas using 15848-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15848-1-AP (INS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 15848-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15848-1-AP (INS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 15848-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 15848-1-AP (INS antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 15848-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 15848-1-AP (INS antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 15848-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas using 15848-1-AP (INS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse pancreas using 15848-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas using 15848-1-AP (INS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 15848-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15848-1-AP (INS antibody) at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human pancreas using 15848-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15848-1-AP (INS antibody) at dilution of 1:1000 (under 40x lens).

• 

  IF Staining of human pancreas using 15848-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 15848-1-AP (INS antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The α cells of the islets of Langerhans was labelled in green with 67286-1-Ig (Glucagon antibody).

• 

  IF Staining of mouse pancreas using 15848-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 15848-1-AP (INS antibody), at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The α cells of the islets of Langerhans was labelled in green with CL488-67286.

• 

  IF Staining of mouse pancreas using 15848-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using INS antibody (15848-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pancreas using 15848-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using INS antibody (15848-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat pancreas using 15848-1-AP

  IF result of Insulin antibody (15848-1-AP, 1:500) with Rat pancreas.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe, humanes Pankreasgewebe, Ratten-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Pankreasgewebe, Maus-Pankreasgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 38 publications below
• IF: See 38 publications below
• ELISA: See 1 publications below

Produktinformation

15848-1-AP bindet in IHC, IF-P, ELISA INS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INS fusion protein Ag8630
• Vollständiger Name: INS
• Berechnetes Molekulargewicht: 110 aa, 12 kDa
• GenBank-Zugangsnummer: BC005255
• Gene symbol: INS
• Gene ID (NCBI): 3630
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus.

Publikationen

Species	Application	Title
mouse	IF	Cell; Long-Term Expansion of Pancreatic Islet Organoids from Resident Procr+ Progenitors.; Authors - Daisong Wang
mouse	IF	Nat Protoc; Isolation of mouse pancreatic islet Procr+ progenitors and long-term expansion of islet organoids in vitro.; Authors - Jingqiang Wang
mouse	IF	Autophagy; Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo; Authors - Shan-Ying Wu
mouse	IF	Environ Pollut; Low-dose bisphenol AF exerts slight effects on glycolipid metabolism but causes metabolic disorders under the stress of Western diet in mice; Authors - Chengzhe Guo
mouse	IHC	Cell Rep; Spatiotemporal role of SETD2-H3K36me3 in murine pancreatic organogenesis; Authors - Ping Lu
mouse	IHC	Theranostics; Circular RNA circPPM1F modulates M1 macrophage activation and pancreatic islet inflammation in type 1 diabetes mellitus.; Authors - Caiyan Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (05-14-2021)

very good for IF

• Applications: Immunofluorescence

FH

Isha (Verified Customer) (01-06-2021)

Worked really well with mice pancreas. Selectively stained islets beta cells, no background staining

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Pancreas