INSIG1 Polyklonaler Antikörper

INSIG1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 55282-1-AP

Synonyme

CL 6, INSIG 1, INSIG-1, insulin induced gene 1, Insulin induced gene 1 protein

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• 

  WB analysis using 55282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55282-1-AP (INSIG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 55282-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55282-1-AP (INSIG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 55282-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 55282-1-AP (INSIG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 55282-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using INSIG1 antibody (55282-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

55282-1-AP bindet in WB, IHC, IF/ICC, ELISA INSIG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: insulin induced gene 1
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_005542
• Gene symbol: INSIG1
• Gene ID (NCBI): 3638
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Insulin-induced genes (INSIGs), which consist of two isoforms (INSIG1 and INSIG2), are representative endoplasmic reticulum (ER)-resident oxysterol-binding proteins (OSBPs). INSIG1 is part of negative regulatory feedback, acting as a brake on SREBP transcriptional function (PMID: 23919961). INSIG1 also has a secondary function mediating sterolaccelerated proteolytic degradation of HMG CoA reductase, thus impacting the mevalonate pathway. Suppression of INSIG1 function promotes hepatic lipid remodelling and restrains NASH (non-alcoholic steatohepatitis) progression(PMID: 33722690).

Publikationen

Species	Application	Title
		Cell Death Differ; Ubiquitination of NLRP3 by gp78/Insig-1 restrains NLRP3 inflammasome activation.; Authors - Ting Xu
mouse	WB	Front Pharmacol; Acacetin protects against depression-associated dry eye disease by regulating ubiquitination of NLRP3 through gp78 signal; Authors - Mingxia Xie
chicken	WB,IF	Poult Sci; Functional and miRNA regulatory characteristics of INSIG genes highlight the key role of lipid synthesis in the liver of chicken (Gallus gallus); Authors - Yaxin Yue
mouse,human	WB,IF	FASEB J; Dickkopf-1 promotes vascular smooth muscle cell foam cell formation and atherosclerosis development through CYP4A11/SREBP2/ABCA1; Authors - Xiao Li
mouse,human	WB	Metabolism; miR-32-5p induces hepatic steatosis and hyperlipidemia by triggering de novo lipogenesis; Authors - Ya-Di Wang
human	WB	Redox Biol; Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis; Authors - Xue Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zach (Verified Customer) (09-12-2025)

Did not detect endogenous protein but detected transgenically overexpressed ones.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: liver