INTS10 Polyklonaler Antikörper

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• KD/KO Validated

INTS10 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 15271-1-AP

Synonyme

C8orf35, INT10, integrator complex subunit 10, INTS10

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Galerie der Validierungsdaten

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  WB analysis using 15271-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15271-1-AP (INTS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 15271-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 15271-1-AP (INTS10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15271-1-AP

  IP Result of anti-INTS10 (IP:15271-1-AP, 4ug; Detection:15271-1-AP 1:500) with HeLa cells lysate 1080ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Mausnierengewebe, Rattenhodengewebe
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below

Produktinformation

15271-1-AP bindet in WB, IP, ELISA INTS10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INTS10 fusion protein Ag7383
• Vollständiger Name: integrator complex subunit 10
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 70-82 kDa
• GenBank-Zugangsnummer: BC001915
• Gene symbol: INTS10
• Gene ID (NCBI): 55174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII; Authors - Xavier Rambout
human	WB	Mol Cell; Integrator Recruits Protein Phosphatase 2A to Prevent Pause Release and Facilitate Transcription Termination.; Authors - Kai-Lieh Huang
human	WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
human	WB	Nat Commun; INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.; Authors - Kevin Sabath ; KD Validated
human	WB	Nat Commun; DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes.; Authors - Junichi Yamamoto
human	WB	Proc Natl Acad Sci U S A; Molecular basis for the interaction between Integrator subunits IntS9 and IntS11 and its functional importance.; Authors - Yixuan Wu