IQGAP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

IQGAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Rezensionen (3)

Kat-Nr. : 22167-1-AP

Synonyme

HUMORFA01, KIAA0051, p195, Ras GTPase-activating-like protein IQGAP1

---

• 

  WB analysis using 22167-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22167-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 22167-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22167-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 22167-1-AP

  IP result of anti-IQGAP1 (IP:22167-1-AP, 4ug; Detection:22167-1-AP 1:8000) with HeLa cells lysate 1085 ug.

• 

  IHC staining of human breast cancer using 22167-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse kidney using 22167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22167-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22167-1-AP (IQGAP1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse kidney using 22167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 22167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 22167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 22167-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 22167-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22167-1-AP (IQGAP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 22167-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HeLa using 22167-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 22167-1-AP (IQGAP1 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 22167-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IQGAP1 antibody (22167-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of HeLa using 22167-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human IQGAP1 (22167-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen, SGC-7901-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Mammakarzinomgewebe, humanes Kolongewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, A549-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

22167-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA IQGAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IQGAP1 fusion protein Ag17821
• Vollständiger Name: IQ motif containing GTPase activating protein 1
• Berechnetes Molekulargewicht: 189 kDa
• Beobachtetes Molekulargewicht: 190-195 kDa
• GenBank-Zugangsnummer: BC151834
• Gene symbol: IQGAP1
• Gene ID (NCBI): 8826
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IQGAP1 is a member of a family of scaffolding proteins that interact with signaling and structural molecules. It localizes to sites of cell-cell contact in epithelial cells and regulates distinct cellular processes including cell adhesion, cell migration, extracellular signals through interacting with numerous protein. Multiple studies have shown that IQGAP1 is up-regulated in many human malignancies, such as lung cancer, ovarian cancer, colon cancer, breast cancer, melanoma and HCC. And IQGAP1 plays a critical role in cancer cell invasion.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma; Authors - Perla Pucci
human	WB,IP	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu ; KD Validated
human	WB	Theranostics; Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission.; Authors - Hui-Fang Hu
	WB	Oncogene; The scaffold protein IQGAP1 links heat-induced stress signals to alternative splicing regulation in gastric cancer cells.; Authors - Andrada-Maria Birladeanu ; KO Validated
human	WB	Oncotarget; Diet-induced hypercholesterolemia promotes androgen-independent prostate cancer metastasis via IQGAP1 and caveolin-1.; Authors - Hyeongsun Moon
human	WB,IF	Front Cell Infect Microbiol; IQGAP1 Mediates Hcp1-Promoted Escherichia coli Meningitis by Stimulating the MAPK Pathway.; Authors - Mingna Zhao ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

JIN-FENG (Verified Customer) (10-01-2025)

I used the IQGAP1 antibody to screen my CRISPR-generated cells. It produced a highly specific signal, which gave me confidence throughout the screening process.

• Applications: Western Blot, Cell culture
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293 cell line

IQGAP1 Antibody Western Blot,Cell culture validation (1:1000 dilution) in HEK293 cell line (Cat no:22167-1-AP)

FH

Joleen (Verified Customer) (06-16-2019)

The antibody recognizes IQGAP (top band) but also another band at a lower molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

IQGAP1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:22167-1-AP)

FH

Chun-Chih (Verified Customer) (01-31-2019)

Works well in IP at recommended conc. Clear and strong band, 1mg protein lysate per condition.

• Applications: Immunoprecipitation,
• Cell Tissue Type: Human breast cancer cells