IRF1 Polyklonaler Antikörper

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• KD/KO Validated

IRF1 Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Kat-Nr. : 11335-1-AP

Synonyme

IRF 1, IRF1, MAR

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 11335-1-AP

  WB result of IRF1 antibody (11335-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRF1 transfected HL-60 cells.

• 

  WB analysis using 11335-1-AP

  Western blot analysis of HeLa cells, untreated or treated with Human IFN-γ (hIFN-γ) （HZ-1301） (100 ng/ml, 48 hr), using 11335-1-AP (IRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11335-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11335-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11335-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 11335-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11335-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 11335-1-AP

  IP result of anti-IRF1 (IP:11335-1-AP, 4ug; Detection:11335-1-AP 1:300) with HL-60 cells lysate 4400 ug.

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  IHC staining of human breast cancer using 11335-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11335-1-AP (IRF1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11335-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11335-1-AP (IRF1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  FC experiment of Jurkat using 11335-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human IRF1 (11335-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit IFN-gamma behandelte HeLa-Zellen, HL-60-Zellen, Jurkat-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 24 publications below
• IHC: See 5 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

11335-1-AP bindet in WB, IHC, IF, FC, CoIP, ChIP, ELISA IRF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IRF1 fusion protein Ag1879
• Vollständiger Name: IRF 1
• Berechnetes Molekulargewicht: 325 aa, 37 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC009483
• Gene symbol: IRF1
• Gene ID (NCBI): 3659
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.

Publikationen

Species	Application	Title
human	WB	J Immunother Cancer; Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis.; Authors - Weinan Guo
human	WB,IHC,IF	Free Radic Biol Med; IRF1/ZNF350/GPX4-mediated ferroptosis of renal tubular epithelial cells promote chronic renal allograft interstitial fibrosis; Authors - Yao Zhang ; KD Validated
human	WB	Front Immunol; Toxoplasma Effector TgIST Targets Host IDO1 to Antagonize the IFN-γ-Induced Anti-parasitic Response in Human Cells.; Authors - Hironori Bando
human	WB	Cancer Commun (Lond); MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma.; Authors - Qingnan Wu
mouse	IF	Int J Mol Sci; Comprehensive Analysis of PANoptosis-Related Gene Signature of Ulcerative Colitis; Authors - Jun-Meng Wang
rat	WB	Inflammation; Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro; Authors - Kai Li