IRF1 Polyklonaler Antikörper

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• KD/KO Validated

IRF1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (5)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(50)

Kat-Nr. : 11335-1-AP

Synonyme

interferon regulatory factor 1, IRF 1, IRF-1, MAR

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  WB analysis using 11335-1-AP

  Western blot analysis of HeLa cells, untreated or treated with Human IFN-γ (hIFN-γ) （HZ-1301） (100 ng/ml, 48 hr), using 11335-1-AP (IRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 11335-1-AP

  WB result of IRF1 antibody (11335-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IRF1 transfected HL-60 cells.

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  WB analysis using 11335-1-AP

  IFN gamma treated THP-1 cells were subjected to SDS PAGE followed by western blot with 11335-1-AP (IRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 11335-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11335-1-AP (IRF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11335-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed IFN gamma treated HepG2 cells using IRF1 antibody (11335-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of Jurkat using 11335-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human IRF1 (11335-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit IFN-gamma behandelte HeLa-Zellen, HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: IFN gamma treated HepG2 cells
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 44 publications below
• IHC: See 9 publications below
• IF: See 10 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 4 publications below

Produktinformation

11335-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA IRF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IRF1 fusion protein Ag1879
• Vollständiger Name: IRF 1
• Berechnetes Molekulargewicht: 325 aa, 37 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC009483
• Gene symbol: IRF1
• Gene ID (NCBI): 3659
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy; Authors - Chen Yang
	WB	Cancer Commun (Lond); Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies; Authors - Lu Tang
human	WB	Nucleic Acids Res; SMARCA4 regulates inducible BRD4 genomic redistribution coupling intrinsic immunity and plasticity in epithelial injury-repair; Authors - Xiaofang Xu
human	WB	J Immunother Cancer; Mefloquine enhances the efficacy of anti-PD-1 immunotherapy via IFN-γ-STAT1-IRF1-LPCAT3-induced ferroptosis in tumors; Authors - Qian Tao
human	WB	J Immunother Cancer; Nanoparticle delivery of miR-21-3p sensitizes melanoma to anti-PD-1 immunotherapy by promoting ferroptosis.; Authors - Weinan Guo
human	WB	Cell Death Dis; PLA2G7 promotes immune evasion of bladder cancer through the JAK-STAT-PDL1 axis; Authors - Ding Peng