IRF5 Polyklonaler Antikörper

IRF5 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

35

Kat-Nr. : 10547-1-AP

Synonyme

interferon regulatory factor 5, IRF 5, IRF5, SLEB10

---

Galerie der Validierungsdaten

• 

  WB analysis using 10547-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10547-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10547-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10547-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10547-1-AP (IRF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10547-1-AP

  IP Result of anti-IRF5 (IP:10547-1-AP, 4ug; Detection:10547-1-AP 1:300) with HeLa cells lysate 1600ug.

• 

  IP experiment of HeLa using 10547-1-AP

  IP Result of anti-IRF5 (IP:10547-1-AP, 4ug; Detection:10547-1-AP 1:300) with HeLa cells lysate 1600ug.

• 

  IHC staining of human prostate cancer using 10547-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10547-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10547-1-AP (IRF5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10547-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10547-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10547-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10547-1-AP (IRF5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10547-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IRF5 antibody (10547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10547-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IRF5 antibody (10547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 10547-1-AP

  Immunofluorescent analysis of HepG2 cells using 10547-1-AP (IRF5 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10547-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug IRF5 antibody (10547-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, Jurkat-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Produktinformation

10547-1-AP bindet in WB, IP, IHC, IF, FC, ELISA IRF5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IRF5 fusion protein Ag0841
• Vollständiger Name: interferon regulatory factor 5
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC004201
• Gene symbol: IRF5
• Gene ID (NCBI): 3663
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). Alternative splice variant encoding different isoforms exist. Catalog#10547-1-AP is a rabbit polyclonal antibody raised against the C-terminal of human IRF5.

Publikationen

Species	Application	Title
human	IF	Br J Sports Med; Chronic inflammation is a feature of Achilles tendinopathy and rupture.; Authors - Stephanie Georgina Dakin
mouse	WB	J Immunother Cancer; Ccl3 enhances docetaxel chemosensitivity in breast cancer by triggering proinflammatory macrophage polarization.; Authors - Dandan Sheng
rat	WB	Am J Transplant; Characterization of Transfusion-Elicited Acute Antibody-Mediated Rejection in a Rat Model of Kidney Transplantation.; Authors - G Huang
mouse	WB	J Control Release; PEGylation of interleukin-10 improves the pharmacokinetic profile and enhances the antifibrotic effectivity in CCl?-induced fibrogenesis in mice.; Authors - Mattos Adriana A
mouse	IHC	Br J Pharmacol; Ibuprofen and diclofenac treatments reduce proliferation of pancreatic acinar cells upon inflammatory injury and mitogenic stimulation.; Authors - Marta Bombardo
mouse	WB,IF	Biochem Pharmacol; Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.; Authors - Hong Lu

Verwandte Produkte

STAT4 Polyclonal antibody

51070-2-AP

IRF3 Polyclonal antibody

11312-1-AP

TRIM63 Polyclonal antibody

55456-1-AP

ASAH1 Polyclonal antibody

11274-1-AP

IRF7 Polyclonal antibody

22392-1-AP

TNF Alpha Monoclonal antibody

60291-1-Ig