IRF5 Monoklonaler Antikörper

IRF5 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

CloneNo.

3A4A10

Kat-Nr. : 66835-1-Ig

Synonyme

interferon regulatory factor 5, IRF 5, IRF5, SLEB10

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Galerie der Validierungsdaten

• 

  WB analysis of A549 using 66835-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66835-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66835-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66835-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, RAW 264.7-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66835-1-Ig bindet in WB,ELISA IRF5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IRF5 fusion protein Ag5442
• Vollständiger Name: interferon regulatory factor 5
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC004201
• Gene symbol: IRF5
• Gene ID (NCBI): 3663
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). IRF5 is also used as M1 marcrophage lineage marker. Alternative splice variant encoding different isoforms exist.