IRF9 Polyklonaler Antikörper

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• KD/KO Validated

IRF9 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 14167-1-AP

Synonyme

IRF 9, IRF9, ISGF 3 gamma, ISGF3, ISGF3 p48 subunit, ISGF3G, p48

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Galerie der Validierungsdaten

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  WB analysis using 14167-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14167-1-AP (IRF9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14167-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14167-1-AP (IRF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 14167-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14167-1-AP (IRF9 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14167-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14167-1-AP (IRF9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 14167-1-AP

  IP Result of anti-IRF9 (IP:14167-1-AP, 4ug; Detection:14167-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of human cervical cancer using 14167-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14167-1-AP (IRF9 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14167-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14167-1-AP (IRF9 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14167-1-AP

  Immunofluorescent analysis of HepG2 cells, using IRF9 antibody 14167-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Mausherzgewebe, MCF-7-Zellen, Rattenherzgewebe, THP-1-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

14167-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA IRF9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IRF9 fusion protein Ag5365
• Vollständiger Name: IRF 9
• Berechnetes Molekulargewicht: 393 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44-48 kDa
• GenBank-Zugangsnummer: BC035716
• Gene symbol: IRF9
• Gene ID (NCBI): 10379
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IRF9 also named ISGF3 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state.

Publikationen

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse	WB	Cell Rep; miR-342-5p decreases ankyrin G levels in Alzheimer's disease transgenic mouse models.; Authors - Xiaqin Sun
	ChIP	PLoS Pathog; A host cell long noncoding RNA NR_033736 regulates type I interferon-mediated gene transcription and modulates intestinal epithelial anti-Cryptosporidium defense.; Authors - Juan Li
		Front Immunol; PMID: 30158934; Authors - Ming-Hsun Tsai
human	WB	Mol Cancer Ther; JAK2 inhibitor SAR302503 abrogates PD-L1 expression and targets therapy resistant non-small cell lung cancers.; Authors - Sean Pitroda
human	WB, IP, IF	J Immunol; Herpes Simplex Virus Type 2 Inhibits Type I IFN Signaling Mediated by the Novel E3 Ubiquitin Protein Ligase Activity of Viral Protein ICP22.; Authors - Mudan Zhang