ISYNA1 Polyklonaler Antikörper

ISYNA1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14142-1-AP

Synonyme

hINO1, hIPS, Ino1, INOS, IPS, IPS 1, ISYNA1, MI 1 P synthase, MIP synthase

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 14142-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14142-1-AP (ISYNA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14142-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14142-1-AP (ISYNA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 14142-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14142-1-AP (ISYNA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14142-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14142-1-AP (ISYNA1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14142-1-AP

  Immunofluorescent analysis of HepG2 cells, using ISYNA1 antibody 14142-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HepG2-Zellen, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

14142-1-AP bindet in WB, IHC, IF ISYNA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ISYNA1 fusion protein Ag5272
• Vollständiger Name: inositol-3-phosphate synthase 1
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC066902
• Gene symbol: ISYNA1
• Gene ID (NCBI): 51477
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Myo-inositol 3-phosphate synthase (ISYNA1/IP synthase) is a rate-limiting enzyme that catalyzes the first step in the biosynthesis of all inositol containing compounds. It converts glucose 6-phosphate to Myo-inositol 3-phosphate.(PMID:21841945). The native enzyme is typically a homotrimermade up of 68-kDa subunits in mammalian cells and a homotetramer in yeast and plant cells. There are three new isoforms of 62, 43, and 16kDa detected suggesting ISYNA1 is a completely different holoenzyme. But only the brain and testis manifest the 68-kDa isoform, whereas the pancreas has a slightly smaller isoformat 67 kDa. The intestine has a completely unique isoformprofile comprising 62,43, 20kDa isoforms(PMID:19188364).

Publikationen

Species	Application	Title
mouse	WB	Acta Pharmacol Sin; Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathway.; Authors - Jian-Yi Dong
mouse	WB	Front Pharmacol; Naringin Exerts Therapeutic Effects on Mice Colitis: A Study Based on Transcriptomics Combined With Functional Experiments; Authors - Jianyi Dong
rat	WB, IF	Alcohol Clin Exp Res; Ethanol supports macrophage recruitment and reinforces invasion and migration of lewis lung carcinoma.; Authors - Keke Yu
mouse	WB	Mol Reprod Dev; Comparative proteomic analysis of proteins involved in oocyte meiotic maturation in mice.; Authors - Cao Senyang S