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Integrin alpha 1 Polyklonaler Antikörper

Integrin alpha 1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 22146-1-AP

Synonyme

ITGA1, VLA 1, Laminin and collagen receptor, integrin, alpha 1, CD49a



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HepG2-Zellen, HT-29-Zellen, SMMC-7721-Zellen, SW480-Zellen
Erfolgreiche Detektion in IHChumanes Lebergewebe, humanes Tonsillitisgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

22146-1-AP bindet in WB, IHC, IF, ELISA Integrin alpha 1 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Integrin alpha 1 fusion protein Ag17584
Vollständiger Name integrin, alpha 1
Berechnetes Molekulargewicht 1179 aa, 131 kDa
Beobachtetes Molekulargewicht 180 kDa
GenBank-ZugangsnummerBC137121
Gene symbol Integrin alpha 1
Gene ID (NCBI) 3672
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Integrin alpha 1 antibody 22146-1-APProtokoll herunterladen
IHC protocol for Integrin alpha 1 antibody 22146-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IHC

Mediators Inflamm

ITGA1 Promotes Glioma Cell Proliferation and Affects Immune Cell Infiltration in Low-Grade Glioma

Authors - Yanhong Ren
humanWB

Int J Biol Sci

Integrin α1 promotes tumorigenicity and progressive capacity of colorectal cancer.

Authors - Hai Li
  • KD Validated
humanWB

Int J Oncol

Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules

Authors - AMINA BOLATKAN
humanWB

J Ethnopharmacol

A combination index and glycoproteomics-based approach revealed synergistic anticancer effects of curcuminoids of turmeric against prostate cancer PC3 cells.

Authors - Meng-Meng Wei
humanWB,IHC,IF

Int J Oncol

FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins.

Authors - Junfen Xu
ratWB,IF

J Biomech

Integrin endocytosis on elastic substrates mediates mechanosensing.

Authors - Jing Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Boyan (Verified Customer) (04-24-2019)

This antibody labelled a band above 250 kD, which is likely the glycosylated form of Itga1. But, it did not label any band around 170 kD as shown in the data sheet.

  • Applications: Western Blot,
  • Cell Tissue Type: MEF