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Integrin alpha 1 Polyklonaler Antikörper
Integrin alpha 1 Polyklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (2)
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 22146-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, HepG2-Zellen, HT-29-Zellen, SMMC-7721-Zellen, SW480-Zellen | 
| Erfolgreiche Detektion in IHC | humanes Lebergewebe,  humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 | 
| Immunhistochemie (IHC) | IHC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 2 publications below | 
| WB | See 11 publications below | 
| IHC | See 3 publications below | 
| IF | See 3 publications below | 
Produktinformation
22146-1-AP bindet in WB, IHC, IF, ELISA Integrin alpha 1 und zeigt Reaktivität mit human
| Getestete Reaktivität | human | 
| In Publikationen genannte Reaktivität | human, Maus, Ratte | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | Integrin alpha 1 fusion protein Ag17584 | 
| Vollständiger Name | integrin, alpha 1 | 
| Berechnetes Molekulargewicht | 1179 aa, 131 kDa | 
| Beobachtetes Molekulargewicht | 180 kDa | 
| GenBank-Zugangsnummer | BC137121 | 
| Gene symbol | Integrin alpha 1 | 
| Gene ID (NCBI) | 3672 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Integrin alpha 1 antibody 22146-1-AP | Protokoll herunterladen | 
| IHC protocol for Integrin alpha 1 antibody 22146-1-AP | Protokoll herunterladenl | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Mediators Inflamm ITGA1 Promotes Glioma Cell Proliferation and Affects Immune Cell Infiltration in Low-Grade Glioma | ||
| Int J Biol Sci Integrin α1 promotes tumorigenicity and progressive capacity of colorectal cancer.
 | ||
| Int J Oncol Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules | ||
| J Ethnopharmacol A combination index and glycoproteomics-based approach revealed synergistic anticancer effects of curcuminoids of turmeric against prostate cancer PC3 cells. | ||
| Int J Oncol FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins. | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Boyan (Verified Customer) (04-24-2019) | This antibody labelled a band above 250 kD, which is likely the glycosylated form of Itga1. But, it did not label any band around 170 kD as shown in the data sheet. 
 | 







