Integrin Alpha 3 Polyklonaler Antikörper

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• KD/KO Validated

Integrin Alpha 3 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 21992-1-AP

Synonyme

CD49C, FRP 2, Galactoprotein B3, GAP B3, GAPB3, Integrin alpha 3, ITGA3, MSK18, VCA 2, VL3A, VLA 3 subunit alpha, VLA3a

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Galerie der Validierungsdaten

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  WB analysis of A549 using 21992-1-AP

  WB result of Integrin Alpha 3 antibody (21992-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin Alpha 3 transfected A549 cells.

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  WB analysis using 21992-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21992-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21992-1-AP (ITGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 21992-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21992-1-AP (ITGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 21992-1-AP

  IP Result of anti-Integrin alpha-3 (IP:21992-1-AP, 4ug; Detection:21992-1-AP 1:500) with PC-3 cells lysate 1200ug.

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  IHC staining of mouse colon using 21992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 21992-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21992-1-AP (Integrin Alpha 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 21992-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Integrin Alpha 3 antibody (21992-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of PC-3 using 21992-1-AP

  1X10^6 PC-3 cells were stained with 0.2ug Integrin alpha-3 antibody (21992-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, humanes Herzgewebe, humanes Hodengewebe, PC-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen
• Erfolgreiche Detektion in FC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

21992-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Integrin Alpha 3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin Alpha 3 fusion protein Ag17293
• Vollständiger Name: integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
• Berechnetes Molekulargewicht: 1066 aa, 119 kDa
• Beobachtetes Molekulargewicht: 130-160 kDa
• GenBank-Zugangsnummer: BC150190
• Gene symbol: ITGA3
• Gene ID (NCBI): 3675
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ITGA3, also named as MSK18, CD49c, GAPB3 and FRP-2, belongs to the integrin alpha chain family. It is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. ITGA3 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. ITGA3 is highly concentrated in epithelial cells where it strongly adheres to Laminin-5 and Laminin-5 induced rapid adhesion can be blocked by antibodies against the alpha-3 integrin subunit. It can be cleaved into heavy chain(840aa) and light chain(176aa). This antibody can recognize the heavy chain.

Publikationen

Species	Application	Title
human	WB	Front Oncol; High Expression of Integrin α3 Predicts Poor Prognosis and Promotes Tumor Metastasis and Angiogenesis by Activating the c-Src/Extracellular Signal-Regulated Protein Kinase/Focal Adhesion Kinase Signaling Pathway in Cervical Cancer.; Authors - Qiqiao Du
human,mouse	WB,IF	Nat Commun; Integrin α3β1 promotes vessel formation of glioblastoma-associated endothelial cells through calcium-mediated macropinocytosis and lysosomal exocytosis.; Authors - Eunnyung Bae

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