Integrin alpha-5/CD49e Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Integrin alpha-5/CD49e Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 27224-1-AP

Synonyme

Integrin Alpha-5, ITGA5, CD49 antigen-like family member E, CD49e, FNRA

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  WB analysis using 27224-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27224-1-AP

  WB result of Integrin alpha-5 antibody (27224-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin alpha-5 transfected HeLa cells.

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  WB analysis of HAP1 using 27224-1-AP

  WB result of Integrin alpha-5/CD49e antibody (27224-1-AP; 1:20000; room temperature for 1.5 hours) with wild-type and Integrin alpha-5/CD49e knockout HAP1 cells.

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  WB analysis of human placenta using 27224-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 27224-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27224-1-AP (ITGA5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27224-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 27224-1-AP

  IP result of anti-Integrin Alpha 5 (IP:27224-1-AP, 4ug; Detection:27224-1-AP 1:1000) with human placenta tissue lysate 2600 ug.

• 

  IP experiment of human placenta using 27224-1-AP

  IP result of anti-Integrin Alpha 5 (IP:27224-1-AP, 4ug; Detection:27224-1-AP 1:1000) with human placenta tissue lysate 4000 ug.

• 

  IHC staining of human placenta using 27224-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 27224-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, U2OS-Zellen
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

27224-1-AP bindet in WB, IHC, IP, ELISA Integrin alpha-5/CD49e und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin alpha-5/CD49e fusion protein Ag26098
• Vollständiger Name: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Berechnetes Molekulargewicht: 115 kDa
• Beobachtetes Molekulargewicht: 135-150 kDa
• GenBank-Zugangsnummer: NM_002205
• Gene symbol: Integrin alpha 5
• Gene ID (NCBI): 3678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	J Clin Transl Hepatol; A Prognostic Nomogram for Hepatocellular Carcinoma Based on Wound Healing and Immune Checkpoint Genes; Authors - Beiyuan Hu
human	WB	Mol Cancer; ZNF460-mediated circRPPH1 promotes TNBC progression through ITGA5-induced FAK/PI3K/AKT activation in a ceRNA manner; Authors - Chuanpeng Zhang
human	WB	Biomater Res; Targeting Reprogrammed Cancer-Associated Fibroblasts with Engineered Mesenchymal Stem Cell Extracellular Vesicles for Pancreatic Cancer Treatment; Authors - Pengcheng Zhou ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (01-25-2019)

By WB, it labels a band at the expected molecular weight, but this band is very likely not the real protein, because it is increased during adipocyte differentiation, which should be decreased during the process according to literature.By IF, PFA fixation, it labels the protein in the nucleus. But according to literature, it is localised in the cell surface and the Gogli.

• Applications: Western Blot, Immunofluorescence,