Integrin Beta 4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Integrin Beta 4 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 21738-1-AP

Synonyme

CD104, GP150, Integrin beta 4, integrin, beta 4, ITGB4

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Galerie der Validierungsdaten

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  WB analysis using 21738-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21738-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21738-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21738-1-AP

  IP Result of anti-Integrin beta-4 (IP:21738-1-AP, 4ug; Detection:21738-1-AP 1:500) with HeLa cells lysate 2400ug.

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  IHC staining of mouse colon using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21738-1-AP (Integrin Beta 4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A431 using 21738-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 4 antibody (21738-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21738-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Integrin beta-4 antibody (21738-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, A431-Zellen, A549-Zellen, HeLa-Zellen, HT-29-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe, humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

21738-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA Integrin Beta 4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Integrin Beta 4 fusion protein Ag16450
• Vollständiger Name: integrin, beta 4
• Berechnetes Molekulargewicht: 1752 aa, 195 kDa
• Beobachtetes Molekulargewicht: 200-240 kDa
• GenBank-Zugangsnummer: BC126411
• Gene symbol: ITGB4
• Gene ID (NCBI): 3691
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

Publikationen

Species	Application	Title
human,mouse	WB,IHC,CoIP	Oncogene; Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.; Authors - Shaojia Wang ; KD Validated
rat	WB	Phytomedicine; Ganoderma lucidum polysaccharide inhibits HSC activation and liver fibrosis via targeting inflammation, apoptosis, cell cycle, and ECM-receptor interaction mediated by TGF-β/Smad signaling; Authors - Chaojie Chen
human	WB,IHC,IF	Sci Rep; Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma.; Authors - Xiao-Long Li
human	WB	Front Oncol; p63 Directs Subtype-Specific Gene Expression in HPV+ Head and Neck Squamous Cell Carcinoma.; Authors - Alexandra Ruth Glathar
human	WB,IHC	Cell Commun Signal; METTL14-mediated N6-methyladenosine modification of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma.; Authors - Zhuonan Liu ; KD Validated
human	IF	Int J Oncol; FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins.; Authors - Junfen Xu

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