ITIH4 Polyklonaler Antikörper

ITIH4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 24069-1-AP

Synonyme

Gp120, H4P, IHRP, ITI HC4, ITI heavy chain H4, ITIH4, ITIHL1, PK 120, PK120

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 24069-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 24069-1-AP (ITIH4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 24069-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 24069-1-AP (ITIH4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 24069-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24069-1-AP (ITIH4 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

24069-1-AP bindet in WB, IP, IHC, IF, ELISA ITIH4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ITIH4 fusion protein Ag21275
• Vollständiger Name: inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
• Berechnetes Molekulargewicht: 103 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC136393
• Gene symbol: ITIH4
• Gene ID (NCBI): 3700
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	IHC	Front Oncol; Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma.; Authors - Changhong Yang
mouse	WB	Clin Exp Immunol; Citrullinated inter-alpha-trypsin inhibitor heavy chain 4 in arthritic joints and its potential effect in the neutrophil migration.; Authors - Atsumu Osada
	WB,IHC	BMC Cancer; Elevated levels of circulating ITIH4 are associated with hepatocellular carcinoma with nonalcoholic fatty liver disease: from pig model to human study.; Authors - Naohiko Nakamura
human	IHC	Dis Markers; Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer.; Authors - Xiao Jiang
mouse	IHC,IF	J Alzheimers Dis; Acute Anti-Inflammatory Markers ITIH4 and AHSG in Mice Brain of a Novel Alzheimer's Disease Model.; Authors - Xiaowen Shi