ITM2C Monoklonaler Antikörper

ITM2C Monoklonal Antikörper für FC, IF, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

2E8G11

Kat-Nr. : 60095-1-Ig

Synonyme

BRICD2C, Cerebral protein 14, E25, E25C, integral membrane protein 2C, ITM2C, ITM3, PSEC0047, Transmembrane protein BRI3

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using 60095-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60095-1-Ig (ITM2C antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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  FC experiment of HepG2 using 60095-1-Ig

  1X10^6 HepG2 cells were stained with 0.20ug ITM2C antibody (60095-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

60095-1-Ig bindet in IF, FC, ELISA ITM2C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ITM2C fusion protein Ag4409
• Vollständiger Name: integral membrane protein 2C
• Berechnetes Molekulargewicht: 230aa,26 kDa; 267aa,30 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC025742
• Gene symbol: ITM2C
• Gene ID (NCBI): 81618
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Integral membrane protein 2C (ITM2C) is a type II integral transmembrane protein belonging to a family composed of at least two other members, ITM2A and ITM2B. ITM2C, often called transmembrane protein BRI3, is highly expressed in brain tissue. Yeast two-hybrid screen system revealed that BRI3 could interact with beta-secretase beta-amyloid protein converting enzyme (BACE)1 and the microtubule-destabilizing protein SCG10 (STMN2). It may play a role in TNF-induced cell death and neuronal differentiation. BRI3 was found to inhibit the various processing of amyloid precursor protein (APP) by blocking the access of alpha- and beta-secretases to APP, competitive inhibition of APP processing by BRI3 may provide a new approach to Alzheimer disease (AD) therapy and prevention.

Publikationen

Species	Application	Title
human	FC	NAR Genom Bioinform; Joint single cell DNA-seq and RNA-seq of gastric cancer cell lines reveals rules of in vitro evolution.; Authors - Noemi Andor