Rabbit IgG control Polyklonaler Antikörper
Rabbit IgG control Polyklonal Antikörper für FC, IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
Anwendung
WB, RIP, IP, IHC, FC, CoIP, ChIP, Cell treatment, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 30000-0-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Protein, Kontrolle |
| Erfolgreiche IP | Kontrolle |
| Erfolgreiche Detektion in FC | Jurkat-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 42 publications below |
| IHC | See 8 publications below |
| IP | See 51 publications below |
| FC | See 4 publications below |
| CoIP | See 25 publications below |
| ChIP | See 33 publications below |
| RIP | See 29 publications below |
Produktinformation
30000-0-AP bindet in WB, RIP, IP, IHC, FC, CoIP, ChIP, Cell treatment, ELISA Rabbit IgG control und zeigt Reaktivität mit
| Getestete Reaktivität | |
| In Publikationen genannte Reaktivität | human, Maus, Ratte, Rind |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | k.A. |
| Vollständiger Name | Rabbit IgG control |
| Gene symbol | |
| Gene ID (NCBI) | |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein A affinity purification |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
This product is Normal rabbit IgG (without immunized) which purified with Protein A. Normal Rabbit IgG is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Publikationen
| Species | Application | Title |
|---|---|---|
Drug Resist Updat CHD4 promotes acquired chemoresistance and tumor progression by activating the MEK/ERK axis | ||
Cell Res Intercellular transfer of activated STING triggered by RAB22A-mediated non-canonical autophagy promotes antitumor immunity | ||
ACS Nano Augmenting Intracellular Cargo Delivery of Extracellular Vesicles in Hypoxic Tissues through Inhibiting Hypoxia-Induced Endocytic Recycling | ||
Mol Cell CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity | ||
Mol Cell Interaction of tau with HNRNPA2B1 and N6-methyladenosine RNA mediates the progression of tauopathy. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Siddharth (Verified Customer) (08-02-2023) | Very efficient secondary antibody. Can be used effectively at 1:10,000 dilution.
|
FH Macarena (Verified Customer) (10-07-2022) | very concentrated. Excellent results. Deffinetly use less than you would normally use.
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FH Guo-rong (Verified Customer) (08-30-2022) | It works well as an antibody control in neutralization experiments
|
FH Anita (Verified Customer) (01-04-2022) | Works well as a control for IP
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FH Kenneth (Verified Customer) (02-14-2019) | Great company with Great products. Highly recommended.
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FH Priyanka (Verified Customer) (12-18-2018) |
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FH Seongjin (Verified Customer) (12-14-2018) |
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