Importin Beta Monoklonaler Antikörper

Importin Beta Monoklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1A12B1

Kat-Nr. : 67597-1-Ig

Synonyme

KPNB1, Importin subunit beta-1, Importin subunit beta 1, Importin 90, Impnb

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  WB analysis using 67597-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67597-1-Ig (Importin beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67597-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67597-1-Ig (Importin Beta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67597-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67597-1-Ig (Importin beta antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using 67597-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Importin Beta (67597-1-Ig, Clone:1A12B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: IMR-32 cells, A549-Zellen, Caco-2-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67597-1-Ig bindet in WB, IHC, FC (Intra), ELISA Importin Beta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Importin Beta fusion protein Ag23094
• Vollständiger Name: karyopherin (importin) beta 1
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 95-97 kDa
• GenBank-Zugangsnummer: BC003572
• Gene symbol: KPNB1
• Gene ID (NCBI): 3837
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

Publikationen

Species	Application	Title
		J Transl Med; TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis; Authors - Xiang-Lin Hao