Integrin alpha 6 Monoklonaler Antikörper

Integrin alpha 6 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B12A1

Kat-Nr. : 66906-1-Ig

Synonyme

ITGA6, 2B12A1, CD49 antigen-like family member F, CD49f, Integrin alpha-6

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  WB analysis of A431 using 66906-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66906-1-Ig (Integrin alpha-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of JAR using 66906-1-Ig

  JAR cells were subjected to SDS PAGE followed by western blot with 66906-1-Ig (Integrin Alpha 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 66906-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66906-1-Ig (Integrin Alpha 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, humanes Plazenta-Gewebe, JAR-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66906-1-Ig bindet in WB, ELISA Integrin alpha 6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Integrin alpha 6 fusion protein Ag25866
• Vollständiger Name: integrin, alpha 6
• Berechnetes Molekulargewicht: 1073 aa, 119 kDa
• Beobachtetes Molekulargewicht: 115-120 kDa
• GenBank-Zugangsnummer: BC136456
• Gene symbol: Integrin alpha 6
• Gene ID (NCBI): 3655
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha 6 (ITGA6, also known as CD49f or VLA-6) is a 120-kDa single-pass type 1 transmembrane glycoprotein, which combines with the beta 1 subunit (ITGB1, CD29) or beta 4 subunit (ITGB4, CD104) to form heterodimeric laminin receptors (PMID: 1976638; 2351695). Integrin alpha 6 mediates cell-to-cell and cell-to-stroma adhesion. It has also been found in many different stem cell populations and is involved in homing and connecting stem cells to their niches and regulating self-renewal mechanisms in stem cells (PMID: 28494695).