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Integrin Beta 1 Monoklonaler Antikörper

Integrin Beta 1 Monoklonal Antikörper für FC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A7C9

Kat-Nr. : 66315-1-Ig

Synonyme

CD29, FNRB, GPIIA, Integrin beta 1, Integrin beta-1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HT-1080 cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    WB analysis of HT-1080 using 66315-1-Ig

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    WB analysis of HUVEC using 66315-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    WB analysis of HT-1080 using 66315-1-Ig

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HUVEC cells using Integrin Beta 1 Monoclonal antibody (66315-1-Ig)

    FC experiment of HUVEC using 66315-1-Ig

    1X10^6 HUVEC cells were stained with 0.20 ug Anti-Human Integrin beta-1 (66315-1-Ig, Clone:1A7C9) (red) or 0.20 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone:T1F8D3F10) (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHT-1080.Zellen, HUVEC-Zellen
Erfolgreiche Detektion in FCHUVEC-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:16000
Durchflusszytometrie (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

66315-1-Ig bindet in WB, FC, ELISA Integrin Beta 1 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Integrin Beta 1 fusion protein Ag24476
Vollständiger Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Berechnetes Molekulargewicht 88 kDa
Beobachtetes Molekulargewicht 130 kDa
GenBank-ZugangsnummerBC020057
Gene symbol ITGB1
Gene ID (NCBI) 3688
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Protokolle

Produktspezifische Protokolle
WB protocol for Integrin Beta 1 antibody 66315-1-IgProtokoll herunterladen
FC protocol for Integrin Beta 1 antibody 66315-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Structure

Phosphorylation of RIAM by Src Promotes Integrin Activation by Unmasking the PH Domain of RIAM.

Authors - Eun-Ah Cho
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval - Tris HCl 0.1M ph 9

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish Retina/Brain
Integrin Beta 1 Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina/Brain (Cat no:66315-1-Ig)