Integrin Beta 1/CD29 Monoklonaler Antikörper

Integrin Beta 1/CD29 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1A7C9

Rezensionen (1)

Kat-Nr. : 66315-1-Ig

Synonyme

ITGB1, 1A7C9, CD29, FNRB, Glycoprotein IIa

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• 

  WB analysis of HT-1080 using 66315-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 66315-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 66315-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66315-1-Ig (Integrin beta-1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66315-1-Ig bindet in WB, ELISA Integrin Beta 1/CD29 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Integrin Beta 1/CD29 fusion protein Ag24476
• Vollständiger Name: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC020057
• Gene symbol: Integrin beta 1
• Gene ID (NCBI): 3688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrin beta-1 (ITGB1), also named as CD29, is a 130 kDa single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Publikationen

Species	Application	Title
human	WB	Structure; Phosphorylation of RIAM by Src Promotes Integrin Activation by Unmasking the PH Domain of RIAM.; Authors - Eun-Ah Cho
rat	WB	Front Med (Lausanne); Low hydrostatic pressure promotes functional homeostasis of nucleus pulposus cells through the TRPV4/CRT/FA complex axis; Authors - Junxian Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval - Tris HCl 0.1M ph 9

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish Retina/Brain

Integrin Beta 1/CD29 Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina/Brain (Cat no:66315-1-Ig)