JAB1 Polyklonaler Antikörper

JAB1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 27511-1-AP

Synonyme

COPS5, SGN5, MOV 34, Jun activation domain-binding protein 1, EC:3.4.-.-

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  WB analysis using 27511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27511-1-AP (JAB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 27511-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27511-1-AP (JAB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 27511-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 27511-1-AP (JAB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 27511-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using JAB1 antibody (27511-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 27511-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using JAB1 antibody (27511-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 27511-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human JAB1 (27511-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

27511-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA JAB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JAB1 fusion protein Ag25950
• Vollständiger Name: COP9 constitutive photomorphogenic homolog subunit 5 (Arabidopsis)
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 35-38 kDa
• GenBank-Zugangsnummer: BC001187
• Gene symbol: JAB1
• Gene ID (NCBI): 10987
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,CoIP	Cell Biol Toxicol; The COP9 signalosome stabilized MALT1 promotes Non-Small Cell Lung Cancer progression through activation of NF-κB pathway; Authors - Yinghui Wang ; KD Validated
	WB	Front Oncol; Identification of Key Transcription Factors and Immune Infiltration Patterns Associated With Breast Cancer Prognosis Using WGCNA and Cox Regression Analysis.; Authors - Xin Yin
human	IF	Mol Carcinog; PLEK2 mediates metastasis and invasion via α5-nAChR activation in nicotine-induced lung adenocarcinoma; Authors - Qiang Li
human	WB	Cancer Cell Int; The potential oncogenic and MLN4924-resistant effects of CSN5 on cervical cancer cells.; Authors - Huilin Zhang
mouse	WB	Neuropsychiatr Dis Treat; COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein.; Authors - En Liang
human	WB	bioRxiv; Crosslinking-mediated Interactome Analysis Identified PHD2-HIF1α Interaction Hotspots and the Role of PHD2 in Regulating Protein Neddylation; Authors - Haiping Ouyang