JP45/JSRP1 Polyklonaler Antikörper

JP45/JSRP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10854-1-AP

Synonyme

JSRP1, Junctional-face membrane protein of 45 kDa homolog, JP-45, JP45, JP 45

---

• 

  WB analysis of human skeletal muscle using 10854-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10854-1-AP (JP45/JSRP1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 10854-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10854-1-AP (JP45/JSRP1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10854-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10854-1-AP (JP45/JSRP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10854-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10854-1-AP (JP45/JSRP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10854-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using JP45/JSRP1 antibody (10854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10854-1-AP bindet in WB, IHC, IF/ICC, ELISA JP45/JSRP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: JP45/JSRP1 fusion protein Ag1300
• Vollständiger Name: junctional sarcoplasmic reticulum protein 1
• Berechnetes Molekulargewicht: 36 kDa, 45 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC021201
• Gene symbol: JP45/JSRP1
• Gene ID (NCBI): 126306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

JSRP1 (Junctional sarcoplasmic reticulum protein 1), also known as JP-45, is an integral protein of the junctional face membrane of the skeletal muscle sarcoplasmic reticulum (SR), and interacts with calsequestrin and the skeletal-muscle dihydropyridine receptor Cav1. JP-45 is an important protein for functional expression of voltage-dependent calcium channels. Involvement of JP-45 in the molecular mechanism underlying skeletal muscle excitation-contraction coupling has been reported. Although the predicated molecular weight of JP-45 is 36 kDa, it appears a 45-kDa band in western blotting in most cases.