KANK2 Polyklonaler Antikörper

KANK2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 21733-1-AP

Synonyme

ANKRD25, KANK2, KIAA1518, MXRA3, SIP, SRC 1 interacting protein, SRC1 interacting protein

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Galerie der Validierungsdaten

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  WB analysis using 21733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21733-1-AP (KANK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21733-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21733-1-AP (KANK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 21733-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21733-1-AP (KANK2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21733-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21733-1-AP (KANK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21733-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21733-1-AP (KANK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21733-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using KANK2 antibody (21733-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 21733-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using KANK2 antibody (21733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 B23/NPM1 antibody (CL594-60096, Clone: 4F12A3, red).

• 

  IF Staining of A549 using 21733-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using KANK2 antibody (21733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mauslungengewebe, Mausnierengewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

21733-1-AP bindet in WB, IHC, IF, ELISA KANK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KANK2 fusion protein Ag16416
• Vollständiger Name: KN motif and ankyrin repeat domains 2
• Berechnetes Molekulargewicht: 851 aa, 91 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC098105
• Gene symbol: KANK2
• Gene ID (NCBI): 25959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Involved in transcription regulation by sequestering nuclear receptor coactivators, such as NCOA1, NCOA2 and NCOA3, in the cytoplasm; the function is deregulated by phosphorylation. May be involved in the control of cytoskeleton formation by regulating actin polymerization. Involved in regulation of caspase-independent apoptosis; proposed to sequester AIFM1 in mitochondria and apoptotic stimuli lead to its proteasomal degradation allowing the release of AIFM1 to the nucleus. May be involved in promotion of cell proliferation.

Publikationen

Species	Application	Title
mouse	WB,IF	J Cell Biol; The RNA-binding protein Fus directs translation of localized mRNAs in APC-RNP granules.; Authors - Yasuda Kyota K
human	WB	J Biol Chem; Nephrotic syndrome-associated mutation of KANK2 induces pathologic binding competition with physiological interactor KIF21A.; Authors - Yuqun Xu
mouse	WB	Am J Physiol Cell Physiol; Knockout of the neonatal Fc Receptor (FcRn) in cultured podocytes alters IL-6 signaling and the actin cytoskeleton.; Authors - Pantipa Tonsawan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bryce (Verified Customer) (09-12-2022)

Strong band at expected molecular weight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000