KBTBD2 Monoklonaler Antikörper

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• KD/KO Validated

KBTBD2 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

2A9F3

Kat-Nr. : 67895-1-Ig

Synonyme

BKLHD1, KBTBD2, KIAA1489

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  WB analysis using 67895-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67895-1-Ig (KBTBD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of U2OS using 67895-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67895-1-Ig (KBTBD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67895-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KBTBD2 antibody (67895-1-Ig, Clone: 2A9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

67895-1-Ig bindet in WB, IHC, IF/ICC, ELISA KBTBD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KBTBD2 fusion protein Ag4933
• Vollständiger Name: kelch repeat and BTB (POZ) domain containing 2
• Berechnetes Molekulargewicht: 622 aa, 71 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC047107
• Gene symbol: KBTBD2
• Gene ID (NCBI): 25948
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106).

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Pathol Res Pract; KBTBD2 promotes proliferation and migration of gastric cancer via activating EGFR signaling pathway; Authors - Jishuang Ding ; KD Validated